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ea0059oc3.2 | Obesity & diabetes | SFEBES2018

Glucose regulates pancreatic β cell Ca²⁺ dynamics and connectivity in vivo in the anterior chamber of the mouse eye

Salem Victoria , Suba Kinga , Martin-Alonso Aldara , Fernando Delgadillo Silva Luis , Akhtar Nadeem , Mousavy Neda , Georgiadou Eleni , Gaboriau David , Rothery Stephen , Stylianides Theodorus , Marchett Piero , Briant Linford , Ninov Nikolay , Hodson David , Distaso Walter , Rutter Guy

Background and Aims: β cell connectivity is a feature of pancreatic islets in vitro but its existence in vivo, when innervated and continuously perfused with blood, has not yet been demonstrated. We imaged islets engrafted in the anterior chamber of the mouse eye (ACE) to explore this question.Methods: Mouse (C57BL6, Ins1Cre::GCaM6mf/f) or human islets infected with adenovirus to express GCaMP6m, were engrafted and Ca<su...

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ea0094p65 | Metabolism, Obesity and Diabetes | SFEBES2023

Differential DNA methylation during postnatal development establishes a subpopulation of pancreatic beta cells with expression of the insulin secretion-enhancing gene, neuronatin

Yu Vanessa , Yong Fiona , Marta Angellica , Khadayate Sanjay , Bhattach Supriyo , Chen Keran , Georgiadou Eleni , Parveen Nazia , Marselli Lorella , De Luca Carmela , Suleiman Mara , Whilding Chad , Marchetti Piero , Dhawan Sangeeta , Withers Dominic , Rutter Guy , Millership Steven

Subpopulations of beta cells dictate overall pacing of insulin secretion across the islet and therefore the control of glycaemia. Functional beta cell subpopulations also exist in human islets and have an altered subtype distribution in type 2 diabetes. However, the molecular control of the establishment and maintenance of beta cell hierarchy is poorly understood. We hypothesised that the epigenome would govern this heterogeneity. We have identified a novel subpopu...

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Endocrine Abstracts

Glucose regulates pancreatic β cell Ca²⁺ dynamics and connectivity in vivo in the anterior chamber of the mouse eye

Differential DNA methylation during postnatal development establishes a subpopulation of pancreatic beta cells with expression of the insulin secretion-enhancing gene, neuronatin

BiosciAbstracts

Endocrine Abstracts

Glucose regulates pancreatic β cell Ca2+ dynamics and connectivity in vivo in the anterior chamber of the mouse eye

Differential DNA methylation during postnatal development establishes a subpopulation of pancreatic beta cells with expression of the insulin secretion-enhancing gene, neuronatin

BiosciAbstracts

Glucose regulates pancreatic β cell Ca²⁺ dynamics and connectivity in vivo in the anterior chamber of the mouse eye